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Image Search Results
Journal: Investigative Ophthalmology & Visual Science
Article Title: Mechanism of Action of VP1-001 in cryAB(R120G)-Associated and Age-Related Cataracts
doi: 10.1167/iovs.18-25647
Figure Lengend Snippet: VP1-001 binds to the ACD and full-length α-crystallin, but ent-VP1-001 does not. (A) Measured T m of cryAB ACD (WT) calculated from Boltzmann fits of DSF traces (n = 3). The shaded gray area represents three standard deviations from the DMSO control. ent-VP1-001 does not shift the thermal stability of the ACD, whereas VP1-001 does. (B) Normalized binding (n = 3) to full-length cryAB (R120G) measured by MST. The shaded gray region represents three standard deviations from the DMSO control. Calculated K D values: VP1-001 = 56 ± 7.0 μM; ent-VP1-001 = 370 ± 30 μM. Thus, VP1-001 binds tighter to cryAB than ent-VP1-001 does, consistent with the increased number of predicted contacts in the docking studies. Note that the ent-VP1-001 curve does not fully saturate, so the calculated K D value is only approximate.
Article Snippet: The DSF data was fitted using a
Techniques: Control, Binding Assay